Graphene-based high-efficiency surface-enhanced Raman scattering-active platform for sensitive and multiplex DNA detection

Shijiang He, Keng Ku Liu, Shao Su, Juan Yan, Xiuhai Mao, Dongfang Wang, Yao He, Lain-Jong Li, Shiping Song*, Chunhai Fan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

164 Scopus citations

Abstract

We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination of gold nanoparticles and graphene, the Raman signals of dye were dramatically enhanced by this novel substrate. With the gold nanoparticles, DNA capture probes could be easily assembled on the surface of graphene films which have a drawback to directly immobilize DNA. This platform exhibits extraordinarily high sensitivity and excellent specificity for DNA detection. A detection limit as low as 10 pM is obtained. Importantly, two different DNA targets could be detected simultaneously on the same substrate just using one light source.

Original languageEnglish (US)
Pages (from-to)4622-4627
Number of pages6
JournalAnalytical Chemistry
Volume84
Issue number10
DOIs
StatePublished - May 15 2012

ASJC Scopus subject areas

  • Analytical Chemistry

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