Golgi inheritance under a block of anterograde and retrograde traffic

Clément Nizak, Rachid Sougrat, Florence Jollivet, Alain Rambourg, Bruno Goud, Franck Perez*

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    11 Scopus citations

    Abstract

    In mitosis, the Golgi complex is inherited following its dispersion, equal partitioning and reformation in each daughter cell. The state of Golgi membranes during mitosis is controversial, and the role of Golgi-intersecting traffic in Golgi inheritance is unclear. We have used brefeldin A (BFA) to perturb Golgi-intersecting membrane traffic at different stages of the cell cycle and followed by live cell imaging the fate of Golgi membranes in those conditions. We observed that addition of the drug on cells in prometaphase prevents mitotic Golgi dispersion. Under continuous treatment, Golgi fragments persist throughout mitosis and accumulate in a Golgi-like structure at the end of mitosis. This structure localizes at microtubule minus ends and contains all classes of Golgi markers, but is not accessible to cargo from the endoplasmic reticulum or the plasma membrane because of the continuous BFA traffic block. However, it contains preaccumulated cargo, and intermixes with the reforming Golgi upon BFA washout. This structure also forms when BFA is added during metaphase, when the Golgi is not discernible by light microscopy. Together the data indicate that independent Golgi fragments that contain all classes of Golgi markers (and that can be isolated from other organelles by blocking anterograde and retrograde Golgi-intersecting traffic) persist throughout mitosis.

    Original languageEnglish (US)
    Pages (from-to)284-299
    Number of pages16
    JournalTraffic
    Volume5
    Issue number4
    DOIs
    StatePublished - Apr 1 2004

    Keywords

    • Brefeldin A
    • Golgi apparatus
    • Intracellular membranes
    • Mitosis
    • Organelles

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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