Glycogen metabolism as a marker of astrocyte differentiation

J. F. Brunet, I. Allaman, Pierre Magistretti, L. Pellerin

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Glycogen is a hallmark of mature astrocytes, but its emergence during astrocytic differentiation is unclear. Differentiation of E14 mouse neurospheres into astrocytes was induced with fetal bovine serum (FBS), Leukemia Inhibitory Factor (LIF), or Ciliary Neurotrophic Factor (CNTF). Cytochemical and enzymatic analyses showed that glycogen is present in FBS-or LIF-but not in CNTF-differentiated astrocytes. Glycogenolysis was induced in FBS-and LIF-differentiated astrocytes but glycogen resynthesis was observed only with FBS. Protein targeting to glycogen mRNA expression appeared with glial fibrillary acidic protein and S100Β in FBS and LIF conditions but not with CNTF. These results show that glycogen metabolism constitutes a useful marker of astrocyte differentiation.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Astrocyte differentiation
  • Brain energy metabolism
  • Glycogen
  • Leukemia Inhibitory Factor

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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