Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes

Ying Gu, Guang Hui Liu*, Nongluk Plongthongkum, Christopher Benner, Fei Yi, Jing Qu, Keiichiro Suzuki, Jiping Yang, Weiqi Zhang, Mo Li, Nuria Montserrat, Isaac Crespo, Antonio del Sol, Concepcion Rodriguez Esteban, Kun Zhang, Juan Carlos Izpisua Belmonte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

With defined culture protocol, human embryonic stem cells (hESCs) are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for cardiac disease therapies. In this study, we successfully generated a highly pure population of human cardiomyocytes (hCMs) (>95% cTnT+) from hESC line, which enabled us to identify and characterize an hCM-specific signature, at both the gene expression and DNA methylation levels. Gene functional association network and gene-disease network analyses of these hCM-enriched genes provide new insights into the mechanisms of hCM transcriptional regulation, and stand as an informative and rich resource for investigating cardiac gene functions and disease mechanisms. Moreover, we show that cardiac-structural genes and cardiac-transcription factors have distinct epigenetic mechanisms to regulate their gene expression, providing a better understanding of how the epigenetic machinery coordinates to regulate gene expression in different cell types.

Original languageEnglish (US)
Pages (from-to)59-68
Number of pages10
JournalProtein and Cell
Volume5
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • DNA methylation
  • heart development
  • human cardiomyocyte
  • microarray

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Drug Discovery
  • Cell Biology

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