Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres

Naouel Klibi, Naouel Ben Slimen, Imen Fhoula, Maria López, Karim Ben Slama, Daniele Daffonchio, Abdellatif Boudabous, Carmen Torres, Hadda Ouzari*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.

Original languageEnglish (US)
Pages (from-to)533-537
Number of pages5
JournalMicrobes and Environments
Volume27
Issue number4
DOIs
StatePublished - Dec 1 2012

Keywords

  • Antibiotic resistance
  • Bacteriocins
  • Enterococci
  • MLST
  • PFGE

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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