TY - JOUR
T1 - Genetic Components of Root Architecture Remodeling in Response to Salt Stress
AU - Julkowska, Magdalena
AU - Koevoets, Iko Tamar
AU - Mol, Selena
AU - Hoefsloot, Huub CJ
AU - Feron, Richard
AU - Tester, Mark A.
AU - Keurentjes, Joost J.B.
AU - Korte, Arthur
AU - Haring, Michel A
AU - de Boer, Gert-Jan
AU - Testerink, Christa
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors would like to thank Willem Kruijer from Wageningen University for help with GWAS, Dorota Kawa and Jessica Meyer from University of Amsterdam for their technical support. We thank Dr. Hiroyushi Kasahara (RIKEN Center for Sustainable Resource Science, Yokohama, Japan) for the provided materials. This work was supported by the Netherlands Organisation for Scientific Research (NWO), STW Learning from Nature project 10987 and ALW Graduate Program grant 831.15.004.
PY - 2017/11/7
Y1 - 2017/11/7
N2 - Salinity of the soil is highly detrimental to plant growth. Plants respond by a redistribution of root mass between main and lateral roots, yet the genetic machinery underlying this process is still largely unknown. Here, we describe the natural variation among 347 Arabidopsis thaliana accessions in root system architecture (RSA) and identify the traits with highest natural variation in their response to salt. Salt-induced changes in RSA were associated with 100 genetic loci using genome-wide association studies (GWAS). Two candidate loci associated with lateral root development were validated and further investigated. Changes in CYP79B2 expression in salt stress positively correlated with lateral root development in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots under salt stress, but not in control conditions. By contrast, high HKT1 expression in the root repressed lateral root development, which could be partially rescued by addition of potassium. The collected data and Multi-Variate analysis of multiple RSA traits, available through the Salt_NV_Root App, capture root responses to salinity. Together, our results provide a better understanding of effective RSA remodeling responses, and the genetic components involved, for plant performance in stress conditions.
AB - Salinity of the soil is highly detrimental to plant growth. Plants respond by a redistribution of root mass between main and lateral roots, yet the genetic machinery underlying this process is still largely unknown. Here, we describe the natural variation among 347 Arabidopsis thaliana accessions in root system architecture (RSA) and identify the traits with highest natural variation in their response to salt. Salt-induced changes in RSA were associated with 100 genetic loci using genome-wide association studies (GWAS). Two candidate loci associated with lateral root development were validated and further investigated. Changes in CYP79B2 expression in salt stress positively correlated with lateral root development in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots under salt stress, but not in control conditions. By contrast, high HKT1 expression in the root repressed lateral root development, which could be partially rescued by addition of potassium. The collected data and Multi-Variate analysis of multiple RSA traits, available through the Salt_NV_Root App, capture root responses to salinity. Together, our results provide a better understanding of effective RSA remodeling responses, and the genetic components involved, for plant performance in stress conditions.
UR - http://hdl.handle.net/10754/626155
UR - http://www.plantcell.org/content/early/2017/11/07/tpc.16.00680
UR - http://www.scopus.com/inward/record.url?scp=85040518267&partnerID=8YFLogxK
U2 - 10.1105/tpc.16.00680
DO - 10.1105/tpc.16.00680
M3 - Article
C2 - 29114015
AN - SCOPUS:85040518267
VL - 29
SP - 3198
EP - 3213
JO - The Plant Cell
JF - The Plant Cell
SN - 1040-4651
IS - 12
ER -