Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

Federico Gonzalez, Montserrat Barragan Monasterio, Gustavo Tiscornia, Nuria Montserrat Pulido, Rita Vassena, Laura Batlle Morera, Ignasi Rodriguez Piza, Juan Carlos Izpisua Belmonte

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.

Original languageEnglish (US)
Pages (from-to)8918-8922
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number22
DOIs
StatePublished - Jun 2 2009

Keywords

  • Nonintegrative technique
  • Pluripotency
  • Reprogramming
  • iPS cells

ASJC Scopus subject areas

  • General

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