Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts

A. Raya*, I. Rodríguez-Pizà, B. Arán, A. Consiglio, P. N. Barri, A. Veiga, Juan Carlos Izpisua Belmonte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Human embryonic stem (hES) cells represent a potential source for cell replacement therapy of many degenerative diseases. Most frequently, hES cell lines are derived from surplus embryos from assisted reproduction cycles, independent of their quality or morphology. Here, we show that hES cell lines can be obtained from poor-quality blastocysts with the same efficiency as that obtained from good- or intermediate-quality blastocysts. Furthermore, we show that the self-renewal, pluripotency, and differentiation ability of hES cell lines derived from either source are comparable. Finally, we present a simple and reproducible embryoid body-based protocol for the differentiation of hES cells into functional cardiomyocytes. The five new hES cell lines derived here should widen the spectrum of available resources for investigating the biology of hES cells and advancing toward efficient strategies of regenerative medicine.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume73
DOIs
StatePublished - Dec 1 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts'. Together they form a unique fingerprint.

Cite this