Gene organization of the quail major histocompatibility complex (MhcCoja) class I gene region

Takashi Shiina, Chiori Shimizu, Akira Oka, Yoshika Teraoka, Tadashi Imanishi, Takashi Gojobori, Kei Hanzawa, Seiki Watanabe, Hidetoshi Inoko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Class I genomic clones of the quail (Coturnix japonica) major histocompatibility complex (MhcCoja) were isolated and characterized. Two clusters spanning the 90.8 kilobase (kb) and 78.2 kb class I gene regions were defined by overlapping cosmid clones and found to contain at least twelve class I loci. However, unlike in the chicken Mhc, no evidence for the existence of any Coja class II gene was obtained in these two clusters. Based on comparative analysis of the genomic sequences with those of the cDNA clones. Coja-A, Coja-B, Coja-C, and Coja-D, these twelve loci were assigned to represent one Coja-A gene, two Coja-B genes (Coja-B1 and -B2), four Coja-C genes (Coja-C1-C4), four Coja-D genes (Coja-D1-D4), and one new Coja-E gene. A class I gene-rich segment of 24.6 kb in which five of these genes (Coja-B1, -B2, -D1, -D2 and -E) are densely packed were sequenced by the shotgun strategy. All of these five class I genes are very compact in size [2089 base pairs (bp)-2732 bp] and contain no apparent genetic defect for functional expression. A transporter associated with the antigen processing (TAP) gene was identified in this class I gene-rich segment. These results suggest that the quail class I region is physically separated from the class II region and characterized by a large number of the expressible class I loci (at least seven) in contrast to the chicken Mhc, where the class I and class II regions are not clearly differentiated and only at most three expressed class I loci so far have been recognized.

Original languageEnglish (US)
Pages (from-to)384-394
Number of pages11
JournalImmunogenetics
Volume49
Issue number5
DOIs
StatePublished - Apr 26 1999

Keywords

  • Antigen processing
  • Cell surface
  • Evolution
  • Mhc
  • Molecules
  • Transporters

ASJC Scopus subject areas

  • Immunology
  • Genetics

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