Functionalized Nanostructures: Redox-Active Porphyrin Anchors for Supramolecular DNA Assemblies

Karl Börjesson, Joanna Wiberg, Afaf H. El-Sagheer, Thomas Ljungdahl, Jerker Mårtensson, Tom Brown, Bengt Nordén, Bo Albinsson

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

We have synthesized and studied a supramolecular system comprising a 39-mer DNA with porphyrin-modified thymidine nucleosides anchored to the surface of large unilamellar vesicles (liposomes). Liposome porphyrin binding characteristics, such as orientation, strength, homogeneity, and binding site size, was determined, suggesting that the porphyrin is well suited as a photophysical and redox-active lipid anchor, in comparison to the inert cholesterol anchor commonly used today. Furthermore, the binding characteristics and hybridization capabilities were studied as a function of anchor size and number of anchoring points, properties that are of importance for our future plans to use the addressability of these redox-active nodes in larger DNA-based nanoconstructs. Electron transfer from photoexcited porphyrin to a lipophilic benzoquinone residing in the lipid membrane was characterized by steady-state and time-resolved fluorescence and verified by femtosecond transient absorption. © 2010 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)5037-5046
Number of pages10
JournalACS Nano
Volume4
Issue number9
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint Dive into the research topics of 'Functionalized Nanostructures: Redox-Active Porphyrin Anchors for Supramolecular DNA Assemblies'. Together they form a unique fingerprint.

Cite this