Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in arabidopsis and rice

Aurelie Evrard, Bastiaan O.R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, Alexander A.T. Johnson*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

15 Scopus citations

Abstract

Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.

Original languageEnglish (US)
Title of host publicationPlant Salt Tolerance
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages265-276
Number of pages12
ISBN (Print)9781617799853
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume913
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • Cell type-specific
  • FACS
  • GAL4
  • Protoplast
  • Rice
  • Salinity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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