Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

Maxim Kogan, Tamás Beke-Somfai, Bengt Nordén

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.
Original languageEnglish (US)
Pages (from-to)7356
JournalChemical Communications
Volume47
Issue number26
DOIs
StatePublished - 2011
Externally publishedYes

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