Field and in vitro evidence for in-situ bioremediation using compound-specific 13C/12C ratio monitoring

L. G. Stehmeier*, M. Mc D. Francis, T. R. Jack, E. Diegor, L. Winsor, T. A. Abrajano

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    50 Scopus citations

    Abstract

    This work describes the use of δ13C values of residual hydrocarbons as a method for demonstrating in-situ biodegradation. Microbial growth, hydrocarbon loss and increase in δ13C values were demonstrated in vitro using benzene and styrene as carbon substrates. Isotope evidence of biodegradation were subsequently sought in four field sites contaminated with a wide variety of hydrocarbons. Analysis of residual hydrocarbons in the field indicated that an overall increase in the δ13C generally accompanied loss of hydrocarbons, an observation consistent with in-situ biodegradation. The field samples were analyzed using vapor or soil extracts, and the increases in δ13C were observed using both types of samples. Vapor sampling is of practical interest because stable isotope ratio monitoring of soil vapor could dramatically reduce the number of wells required for monitoring of ongoing remediation efforts. Our preliminary studies of contaminated field sites allude to the potential of compound-specific isotopic monitoring techniques as a cost-effective measure of in-situ biodegradation.

    Original languageEnglish (US)
    Pages (from-to)821-833
    Number of pages13
    JournalOrganic Geochemistry
    Volume30
    Issue number8 A
    DOIs
    StatePublished - Aug 1 1999

    Keywords

    • Aerobic microorganisms, Stable isotope fractionation, Dicyclopentadiene
    • Benzene, Styrene, Toluene, Residual hydrocarbon

    ASJC Scopus subject areas

    • Geochemistry and Petrology

    Fingerprint

    Dive into the research topics of 'Field and in vitro evidence for in-situ bioremediation using compound-specific <sup>13</sup>C/<sup>12</sup>C ratio monitoring'. Together they form a unique fingerprint.

    Cite this