Fabrication of a surface plasmon resonance biosensor based on gold nanoparticles chemisorbed onto a 1,10-decanedithiol self-assembled monolayer

E. M.S. Azzam, A. Bashir, Osama Shekhah, A. R.E. Alawady, A. Birkner, Ch Grunwald*, Ch Wöll

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

We have investigated the fabrication of surface plasmon resonance (SPR) biosensors using self-assembled monolayers (SAMs) and adsorbed gold nanoparticles. The SAM of 1,10-decanedithiol was first fabricated onto a gold substrate. Gold nanoparticles were then chemisorbed onto the SAM surface by bonding with the terminal thiol groups, forming a sensor that can be used to immobilize proteins. Bovine serum albumin (BSA) was used as a test protein in this study. Several spectroscopic and microscopic techniques were used to investigate both the SAM and the chemisorption of gold nanoparticles at the SAM surface. Our results confirm the covalent bonding of the gold nanoparticles onto the SAM. Surface plasmon resonance (SPR) was used to study both the adsorption of BSA to the SAM surface and to the gold nanoparticle-coated SAM. For SAM surfaces with adsorbed gold nanoparticles a larger SPR response to BSA than to the sensors with a bare SAM is observed.

Original languageEnglish (US)
Pages (from-to)387-391
Number of pages5
JournalThin Solid Films
Volume518
Issue number1
DOIs
StatePublished - Nov 2 2009

Keywords

  • Biosensors
  • Gold nanoparticles
  • Metallization
  • Proteins
  • Self-assembled monolayers
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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