Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths

Anna Nordborg, Frantisek Svec*, Jean M.J. Fréchet, Knut Irgum

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 μm ID capillaries and their application for μ-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 μm suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.

Original languageEnglish (US)
Pages (from-to)2401-2406
Number of pages6
JournalJournal of Separation Science
Volume28
Issue number17
DOIs
StatePublished - Nov 2005
Externally publishedYes

Keywords

  • μ-HPLC
  • Capillary
  • Crosslinker
  • Monolith
  • Monolithic column
  • Polymerization
  • Pore size
  • Protein separation
  • Reverse phase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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