Exploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage

Claudius Marondedze, Aloysius Tze Wong, Arnoud Groen, Natalia Lorena Gorron Serano, Boris R. Jankovic, Kathryn Lilley, Christoph A Gehring, Ludivine Thomas

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The study of proteomes provides new insights into stimulus-specific responses of protein synthesis and turnover, and the role of post-translational modifications at the systems level. Due to the diverse chemical nature of proteins and shortcomings in the analytical techniques used in their study, only a partial display of the proteome is achieved in any study, and this holds particularly true for plant proteomes. Here we show that different solubilization and separation methods have profound effects on the resulting proteome. In particular, we observed that the type of detergents employed in the solubilization buffer preferentially enriches proteins in different functional categories. These include proteins with a role in signaling, transport, response to temperature stimuli and metabolism. This data may offer a functional bias on comparative analysis studies. In order to obtain a broader coverage, we propose a two-step solubilization protocol with first a detergent-free buffer and then a second step utilizing a combination of two detergents to solubilize proteins.
Original languageEnglish (US)
Pages (from-to)857-870
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume16
Issue number1
DOIs
StatePublished - Dec 31 2014

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