Exploring neural cell dynamics with digital holographic microscopy

Pierre Marquet, Christian D. Depeursinge, Pierre J. Magistretti

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological ormation provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach. Copyright © 2013 by Annual Reviews.
Original languageEnglish (US)
Pages (from-to)407-431
Number of pages25
JournalAnnual Review of Biomedical Engineering
Volume15
Issue number1
DOIs
StatePublished - Jul 11 2013

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)

Fingerprint

Dive into the research topics of 'Exploring neural cell dynamics with digital holographic microscopy'. Together they form a unique fingerprint.

Cite this