Exploration of ionic modification in dual-lifer hollow fiber membranes for long-term high-performance protein separation

Yi Li, Sim Chuan Soh, Tai-Shung Chung*, Sui Yung Chan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Two types of ionic modification approaches (i.e., sulfonation and triethylamination) were applied with the aid of dual-layer hollow fiber technology in this work to fine tune the pore size and pore size distribution, introduce the electrostatic interaction, and reduce membrane fouling for long-term high-performance protein separation. A binary protein mixture comprising bovine serum albumin (BSA) and hemoglobin (Hb) was separated in this work. The sulfonated fiber exhibits an improved BSA/Hb separation factor at pH = 6.8 compared with as-spun fibers hut at the expense of BSA sieving coefficient. On the other hand, the triethylaminated fiber reveals the best and most durable separation performance at pH = 4.8. Its BSA/Hb separation factor is maintained above 80 for 4 days and maximum BSA sieving coefficient reaches 33%. Therefore, this study documents that an intelligent combination of both size-exclusion and electrostatic interaction can synergistically enhance protein separation performance in both purity and concentration.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalAIChE Journal
Volume55
Issue number2
DOIs
StatePublished - Feb 1 2009

Keywords

  • Dual-layer hollow fiber membranes
  • Electrostatic interaction
  • Ionic modification
  • Protein separation
  • Size-exclusion

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Biotechnology
  • Environmental Engineering

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