Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9

Christian Frøkjær-Jensen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

With remarkable speed, the CRISPR-Cas9 nuclease has become the genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because the Cas9 nuclease can be guided by a single, short RNA (sgRNA) to introduce double-strand DNA breaks (DSBs) at precise locations. Here I contrast and highlight protocols recently developed by eight different research groups, six of which are published in GENETICS, to modify the Caenorhabditis elegans genome using CRISPR/Cas9. This reverse engineering tool levels the playing field for experimental geneticists.

Original languageEnglish (US)
Pages (from-to)635-642
Number of pages8
JournalGenetics
Volume195
Issue number3
DOIs
StatePublished - Nov 11 2013

ASJC Scopus subject areas

  • Genetics

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