Evidence for the activiation of a MAP kinase upon phosphate-induced cell cycle re-entry in tobacco cells

Cathal Wilson, Martin Pfosser, Claudia Jonak, Heribert Hirt, Erwin Heberle-Bors, Oscar Vicente*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Mitogen-activated-protein (MAP) kinases are components of signal transduction pathways which respond to a variety of stimuli in different organisms. In quiescent mammalian cells, the reactivation of cell division induced by different mitogenic signals is mediated by the rapid phosphorylation and activation of MAP kinases. We have investigated whether a similar situation occurs in plants, arresting tobacco (Nicotiana tobacum L.) cells in the G 1 phase of the cell by phosphate starvation, and then inducing them to re-enter the cell cycle by refeeding with phosphate. The transcient activation of a kinase activity with the characteristics of a MAP kinase was observed during the first hour after refeeding, when the cells were still in G 1 . Using myelin basic protein (MBP) as substrate, an increase in this phosphorylating activity, with a molecular mass of approximately 45 kDa, was detected in cell extracts between 35 and 55 min after induction, in in-gel phosphorylation assays and after immunoprecipitation with anti-MAP kinase antibodie. The specificity of the antibodies against recombinant tobacco MAP kinases suggested that the MAP kinase p 45 (nif4) was responsible for the observed activity. These data provide experimental evidence for the activation in vivo of a plant MAP kinase, possibly mediating the reactivation of cell division in G 1 -arrested cells.

Original languageEnglish (US)
Pages (from-to)532-538
Number of pages7
JournalPhysiologia Plantarum
Volume102
Issue number4
DOIs
StatePublished - Apr 1 1998

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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