Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Noriko Karasaki, Hiroaki Mon, Masateru Takahashi, Jae Man Lee, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    8 Scopus citations

    Abstract

    Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.

    Original languageEnglish (US)
    Pages (from-to)495-500
    Number of pages6
    JournalBiotechnology Letters
    Volume31
    Issue number4
    DOIs
    StatePublished - Apr 1 2009

    Keywords

    • Bombyx mori
    • Doxycycline
    • RtTA
    • Tet-on system
    • TetO

    ASJC Scopus subject areas

    • Biotechnology

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