Erythrocytes volume and refractive index measurement with a digital holographic microscope

B. Rappaz*, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge, P. Magistretti

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

8 Scopus citations

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm3.

Original languageEnglish (US)
Title of host publicationOptical Diagnostics and Sensing VII
Volume6445
DOIs
StatePublished - 2007
Externally publishedYes
EventOptical Diagnostics and Sensing VII - San Jose, CA, United States
Duration: Jan 23 2007Jan 24 2007

Other

OtherOptical Diagnostics and Sensing VII
CountryUnited States
CitySan Jose, CA
Period01/23/0701/24/07

Keywords

  • Cell imaging
  • Digital holography
  • Erythrocyte
  • Refractive index

ASJC Scopus subject areas

  • Engineering(all)

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