Enzyme microarrays: On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity

Jorg Eppinger*, Daniel P. Funeriu, Masato Miyake, Lucile Denizot, Jun Miyake

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

High-throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained "on-chip" by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture).

Original languageEnglish (US)
Pages (from-to)3806-3810
Number of pages5
JournalAngewandte Chemie - International Edition
Volume43
Issue number29
DOIs
StatePublished - Jul 19 2004

Keywords

  • Enzymes
  • Fluorescent probes
  • High-throughput screening
  • Inhibitors
  • Microarrays
  • On-chip kinetics

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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