Die sexualsteroid-vermittelte verstärkung liposomaler transfektion gynäkologischer krebszellen: Ein neuer ansatz für ein selektives targeting in der gentherapie?

Translated title of the contribution: Enhanced liposomal transfection through the application of sexsteroids in gynecological cancer cells

F. Köster, D. Finas, A. Saupp, C. Schulz, K. Diedrich, C. Hauser, Ricardo Felberbaum*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Objective: Liposomal transfection in gene therapeutic application against gynecological malignoma does not reach satisfying efficacy. A desirable goal would be the specific intensification of transfection in these kind of cells. Steroids have successfully been used in other systems to increase liposomal transfection and hopefully there might be a specific impact of sexual steroids in cells from high sex steroid receptor expressing malignoma, like some mamma- and endometrium cancer. Material and Methods: The mamma carcinoma cell line T-47D was transfected with the transfection agent DOTAP and cyclodextrin solubilized steroids and cholesterol were co-applied. The efficiency of transfection was followed by luciferase activity resulting from the transfected reporter gene. Results: Like cholesterol, which is already established as transfection co-agent, also the steroids progesterone, estrogen, testosterone and hydrocortisone provoked a clear increase in transfection efficiency shown in a dose dependent manner. Conclusions: These results indicate the usefulness of steroids as additives for liposomal transfection procedures in gene therapeutic application. As sexual steroid receptors migrate into the nucleus of a cell after binding its specific ligand a targeted enhancement of transfection is supposable in malignoma overexpressing steroid receptors. There is evidence that plasmid DNA can be co-transported with nuclear proteins into the nucleus.

Translated title of the contributionEnhanced liposomal transfection through the application of sexsteroids in gynecological cancer cells
Original languageGerman
Pages (from-to)1-5
Number of pages5
JournalZentralblatt fur Gynakologie
Volume125
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Liposomal transfection
  • Sexual steroids
  • T-47D human breast cancer cell line

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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