Electrical characteristics of the tonoplast of cham corallincr. A study using permeabilised cells

Mark Tester*, Mary J. Beilby, Teruo Shimmen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Use of permeabilised cells of Chara corallina provides a unique opportunity to study the electrical characteristics of the tonoplast whilst being able to control ionic conditions on the outside of the membrane. Current-voltage (I/V) analysis over wide voltage spans, and admittance measurements at 5 Hz showed that many permeabilised cells had a similar conductance and capacitance to the tonoplast of intact cells. Cells developed two regions of negative-slope conductance upon addition of external Cl-, which suggests the existence of potential-dependent Cl- channels in the Chara tonoplast. With Cl- concentrations similar to those expected in vivo, the resting potential was more sensitive to changes in external K+ than Cl-; however, a decrease in external K+ did not significantly alter the shape of the I/V relation.

Original languageEnglish (US)
Pages (from-to)1555-1568
Number of pages14
JournalPlant and Cell Physiology
Volume28
Issue number8
DOIs
StatePublished - Jan 1 1987

Keywords

  • Admittance
  • Charophyte
  • Cl- channel
  • Current-voltage (I/V)
  • Membrane capacitance
  • Voltage clamp.

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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