Efficacy of clone fingerprinting methodologies

William M. Nelson, Jan Dvorak, Ming Cheng Luo, Joachim Messing, Rod A. Wing, Carol Soderlund

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

With the development of new high-information content fingerprinting techniques for constructing BAC-based physical maps, physical map construction is accelerating and it is important to determine which methodologies work best. In a recent publication (Z. Xu et al., 2004, Genomics 84:941-951), Xu et al. evaluated five different techniques (one agarose-based and four using multiple enzymes) and concluded that a two-enzyme technique was superior. In addition, they found that no benefit was gained from fingerprinting more than 10× coverage. In this paper we report our own extensive simulation results, which lead to contrasting conclusions. Our data indicate that the five-enzyme method known as SNaPshot is the most effective and that the assembly can in fact be significantly improved with greater than 10× coverage. © 2006 Elsevier Inc. All rights reserved.
Original languageEnglish (US)
JournalGenomics
Volume89
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

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