Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)

Matteo Montagna, Bessem Chouaia, Giuseppe Mazza, Erica Maria Prosdocimi, Elena Crotti, Valeria Mereghetti, Violetta Vacchini, Annamaria Giorgi, Alessio De Biase, Santi Longo, Rita Cervo, Giuseppe Carlo Lozzia, Alberto Alma, Claudio Bandi, Daniele Daffonchio

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Rhynchophorus ferrugineus, also known as the red palm weevil, is regarded as the major pest of palm trees. Although studies of the microbiota associated with this species have been performed in recent years, little attention has been dedicated to the influence of the diet in shaping the host bacterial community. Here, we investigated the influence of food sources (i.e. palm tissues vs apple based substrate) on the microbial diversity associated with RPW, which was compared with the microbiota associated with wild individuals of the sister species Rhynchophorus vulneratus. The bacterial characterization was performed using a culture independent approach, i.e. the 16S rRNA pyrotag, and a culture dependent approach for a subset of the samples, in order to obtain bacterial isolates from RPW tissues. The bacterial community appeared significantly influenced by diet. Proteobacteria resulted to be the most abundant clade and was present in all the specimens of the three examined weevil groups. Within Proteobacteria, Enterobacteriaceae were identified in all the organs analysed, including hemolymph and reproductive organs. The apple-fed RPWs and the wild R. vulneratus showed a second dominant taxon within Firmicutes that was scarcely present in the microbiota associated with palm-fed RPWs. A comparative analysis on the bacteria associated with the palm tissues highlighted that 12 bacterial genera out of the 13 identified in the plant tissues were also present in weevils, thus indicating that palm tissues may present a source for bacterial acquisition.
Original languageEnglish (US)
Pages (from-to)e0117439
JournalPLoS ONE
Volume10
Issue number1
DOIs
StatePublished - Jan 30 2015

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