Effects of ethanol on VIP- and/or noradrenaline-stimulated cAMP formation in mouse brain

Nicolas C. Schaad*, Pierre Magistretti, Michel Schorderet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as shown in potentiating, at toxic concentrations, the stimulating effect of hormones or neurotransmitters. We have also found that EtOH potentiates in a dose-dependent manner (EC50=100 mM) the cAMP production elicited by vasoactive intestinal peptide (VIP), already noticeably at 70 mM, without affecting basal cAMP levels (up to 400 mM). Propanol produces a similar potentiation, whereas methanol was inactive. Butanol (200 mM) displays toxic effects. The potentiation induced by EtOH is similar for peptide- (VIP) or monoamine- (noradrenaline) stimulated cAMP formation, suggesting a primary action at a level different from the receptor recognition sites. Finally, we have observed that EtOH potentiates the synergistic interaction between VIP and NA in stimulating cAMP formation.

Original languageEnglish (US)
Pages (from-to)445-449
Number of pages5
JournalAlcohol
Volume5
Issue number6
DOIs
StatePublished - Jan 1 1988

Keywords

  • Adenylate cyclase
  • Ethanol
  • Noradrenaline
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Neuroscience(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

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