Effects of auxin and abscisic acid on cytosolic calcium and pH in plant cells

Christoph Gehring, H. R. Irving, R. W. Parish

Research output: Contribution to journalArticlepeer-review

188 Scopus citations

Abstract

Dark-grown corn coleoptiles and parsley hypocotyls and their roots were loaded with acetoxymethyl esterified forms of the Ca2+ indicator fluo-3, and the pH indicator 2',7'-bis(2-carboxyethyl)-5(and -6)-carboxyfluorescein. These tissues were treated with the plant growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D), an auxin analogue, or abscisic acid (ABA), and the cytosolic pH (pH(cyt)) and cytosolic Ca2+ ([Ca2+](cyt)) changes were monitored by confocal scanning optical microscopy. Over a period of 4 min pH(cyt) decreased 0.1-0.2 pH unit and [Ca2+](cyt) increased from 280 to 380 nM in response to 2,4-D. ABA, on the other hand, induced cytosolic alkalinization of 0.05-0.1 pH unit with a concomitant increase in [Ca2+](cyt) from 240 to 320 nM over a 4-min period. Responses similar to these were observed in all the tissues tested. We suggest that pH(cyt) profoundly influences signaling by [Ca2+](cyt), possibly by regulating Ca2+-protein binding, and that the divergent effects of auxin and ABA on pH(cyt) underlie their mutual antagonism.

Original languageEnglish (US)
Pages (from-to)9645-9649
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number24
DOIs
StatePublished - Dec 1 1990

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • confocal scanning optical microscopy
  • plant hormones

ASJC Scopus subject areas

  • General

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