Effect of the spraying conditions and nozzle design on the shape and size distribution of particles obtained with supercritical fluid drying

Andréanne Bouchard*, Nataša Jovanović, Anne H. de Boer, Ángel Martín, Wim Jiskoot, Daan J A Crommelin, Gerard W. Hofland, Geert Jan Witkamp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In the perspective of production of dry therapeutic protein formulations, spray drying of lysozyme (as a model protein) into supercritical carbon dioxide was studied. The effects of the nozzle (i.e., co-current coaxial converging and converging-diverging, and T-mixer impinging) and process conditions (i.e., flow rates, pressure) on the drying of the lysozyme prepared in aqueous solution dried with supercritical carbon dioxide enriched with ethanol were investigated. The particle size distribution, width of particle size distribution and morphology were used to determine the effect of the various parameters assessed. Particles with a median size of ∼1.5, ∼5 or ∼25 μm were produced depending of the nozzle selected. A basic comparative study of the nozzle was done by computational fluid dynamics, but the differences in particle size could not be depicted by these computations. The proportional increase of the flow rates (up to fivefold) caused a decrease in particle size (7- to 12-fold), and doubling the pressure caused a moderate decrease of the size (5-20%). The individual effect of the supercritical carbon dioxide, ethanol and solution streams was explained with a mass transfer model. Changing the ratio between flow rates slightly affected the particle size in various ways because of the swelling and shrinking stages of the drying droplet in supercritical carbon dioxide enriched with ethanol.

Original languageEnglish (US)
Pages (from-to)389-401
Number of pages13
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume70
Issue number1
DOIs
StatePublished - Sep 1 2008
Externally publishedYes

Keywords

  • Atomisation
  • Computational fluid dynamics
  • Lysozyme
  • Mass transfer
  • Particle size distribution

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science

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