Effect of porosity and surface chemistry on the characterization of synthetic polymers by HPLC using porous polymer monolithic columns

Miroslav Jancǒ, Shaofeng Xie, Dominic S. Peterson, Robert W. Allington, Frantisek Svec*, Jean Frechet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Monolithic 50 × 4.6 mm ID poly(styrene-co-divinylbenzene), poly(glycidyl methacrylate-co-ethylene dimethacrylate), and poly(2,3-dihydroxypropyl methacrylate-co-ethylene dimethacrylate) columns have been tested in the high-performance liquid chromatography (HPLC) separation of polystyrenes and poly(methyl methacrylate) standards. The separation process involves precipitation of the macromolecules on the macroporous monolithic column followed by progressive redissolution and elution utilizing a gradient of the mobile phase. While very good separations of individual components of mixtures of both polystyrenes and poly(methyl methacrylates) can be readily achieved using poly(styrene-co-divinylbenzene) monolith, methacrylate-based columns do not afford good separations under identical conditions. This unexpected finding appears to be the result of significant differences between the porous properties and chemistries of the two types of monolithic materials.

Original languageEnglish (US)
Pages (from-to)909-916
Number of pages8
JournalJournal of Separation Science
Volume25
Issue number14
DOIs
StatePublished - Oct 1 2002

Keywords

  • Gradient elution
  • HPLC
  • Molecular weight
  • Monolithic column
  • Polymers
  • Synthetic polymers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Fingerprint

Dive into the research topics of 'Effect of porosity and surface chemistry on the characterization of synthetic polymers by HPLC using porous polymer monolithic columns'. Together they form a unique fingerprint.

Cite this