Dynamics of intracellular oxygen in PC12 cells upon stimulation of neurotransmission

Alexander V. Zhdanov, Manus Ward, Jochen H.M. Prehn, Dmitri B. Papkovsky

    Research output: Contribution to journalArticlepeer-review

    32 Scopus citations

    Abstract

    Neurotransmission, synaptic plasticity, and maintenance of membrane excitability require high mitochondrial activity in neurosecretory cells. Using a fluorescence-based intracellular O 2 sensing technique, we investigated the respiration of differentiated PC12 cells upon depolarization with 100mMK + . Single cell confocal analysis identified a significant depolarization of the plasma membrane potential and a relatively minor depolarization of the mitochondrial membrane potential following K + exposure. We observed a two-phase respiratory response: a first intense spike lasting ∼10 min, during which average intracellular O 2 was reduced from 85-90% of air saturation to 55-65%, followed by a second wave of smaller amplitude and longer duration. The fast rise in O 2 consumption coincided with a transient increase in cellular ATP by ∼60%, which was provided largely by oxidative phosphorylation and by glycolysis. The increase of respiration was orchestrated mainly by Ca 2+ release from the endoplasmic reticulum, whereas the influx of extracellular Ca 2+ contributed ∼20%. Depletion of Ca 2+ stores by ryanodine, thapsigargin, and 4-chloro-m-cresol reduced the amplitude of respiratory spike by 45, 63, and 71%, respectively, whereas chelation of intracellular Ca 2+ abolished the response. Uncoupling of the mitochondria with the protonophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone amplified the responses to K + ; elevated respiration induced a profound deoxygenation without increasing the cellular ATP levels reduced by carbonyl cyanide p-trifluoromethoxyphenylhydrazone. Cleavage of synaptobrevin 2 by tetanus toxin, known to reduce neurotransmission, did not affect the respiratory response to K + , whereas the general excitability of d PC12 cells increased.

    Original languageEnglish (US)
    Pages (from-to)5650-5661
    Number of pages12
    JournalJournal of Biological Chemistry
    Volume283
    Issue number9
    DOIs
    StatePublished - Feb 29 2008

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Dynamics of intracellular oxygen in PC12 cells upon stimulation of neurotransmission'. Together they form a unique fingerprint.

    Cite this