Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator

David Latrasse, Teddy Jégu, Pin Hong Meng, Christelle Mazubert, Elodie Hudik, Marianne Delarue, Céline Charon, Martin Crespi, Heribert Hirt, Cécile Raynaud, Catherine Bergounioux, Moussa Benhamed*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Because regulation of its activity is instrumental either to support cell proliferation and growth or to promote cell death, the universal myo-inositol phosphate synthase (MIPS), responsible for myo-inositol biosynthesis, is a critical enzyme of primary metabolism. Surprisingly, we found this enzyme to be imported in the nucleus and to interact with the histone methyltransferases ATXR5 and ATXR6, raising the question of whether MIPS1 has a function in transcriptional regulation. Here, we demonstrate that MIPS1 binds directly to its promoter to stimulate its own expression by locally inhibiting the spreading of ATXR5/6-dependent heterochromatin marks coming from a transposable element. Furthermore, on activation of pathogen response, MIPS1 expression is reduced epigenetically, providing evidence for a complex regulatory mechanism acting at the transcriptional level. Thus, in plants, MIPS1 appears to have evolved as a protein that connects cellular metabolism, pathogen response and chromatin remodeling.

Original languageEnglish (US)
Pages (from-to)2907-2917
Number of pages11
JournalNucleic acids research
Volume41
Issue number5
DOIs
StatePublished - Mar 1 2013

ASJC Scopus subject areas

  • Genetics

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