Do plasma proteins distinguish between liposomes of varying charge density?

Anna Laura Capriotti, Giulio Caracciolo, Chiara Cavaliere, Patrizia Foglia, Daniela Pozzi, Roberto Samperi, Aldo Laganà

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Cationic liposomes (CLs) are one of the most employed nonviral nanovector systems in gene therapy. However, their transfection efficiency is strongly affected by interactions with plasma components, that lead to the formation of a "protein corona" onto CL surface. The interactions between nanoparticles entering the body and biomolecules have an essential role for their biodistribution. Because the knowledge of proteins adsorbed onto vector surface could be useful in the screening of new, more efficient and more biocompatible liposomal formulations, the behavior of three CLs with different membrane charge densities was investigated. The proteins of the three coronas were identified by nano-liquid chromatography-tandem mass spectrometry, and quantified with label-free spectral counting strategy. Fibrinogen displayed higher association with CLs with high membrane charge density, while apolipoproteins and C4b-binding protein with CLs with low membrane charge density. These results are discussed in terms of the different lipid compositions of CLs and may have a deep biological impact for in vivo applications. Surface charge of nanoparticles is emerging as a relevant factor determining the corona composition after interaction with plasma proteins. Remarkably, it is also shown that the charge of the protein corona formed around CLs is strongly related to their membrane charge density. © 2012 Elsevier B.V.
Original languageEnglish (US)
Pages (from-to)1924-1932
Number of pages9
JournalJournal of Proteomics
Volume75
Issue number6
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Dive into the research topics of 'Do plasma proteins distinguish between liposomes of varying charge density?'. Together they form a unique fingerprint.

Cite this