DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

Peigang Deng, Donald C. Chang, Yi-Kuen Lee, Junwei Zhou, Gang Li

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    4 Scopus citations

    Abstract

    Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.
    Original languageEnglish (US)
    Title of host publication2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
    PublisherInstitute of Electrical and Electronics Engineers (IEEE)
    Pages96-99
    Number of pages4
    ISBN (Print)9781612847757
    DOIs
    StatePublished - Feb 2011

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