DNA skybridge: 3D structure producing a light sheet for high-throughput single-molecule imaging.

Daehyung Kim, Fahad Rashid, Yeonmo Cho, Manal Zaher, I I Hwan Cho, Samir Hamdan, Cherlhyun Jeong, Jong-Bong Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Real-time visualization of single-proteins or -complexes on nucleic acid substrates is an essential tool for characterizing nucleic acid binding proteins. Here, we present a novel surface-condition independent and high-throughput single-molecule optical imaging platform called 'DNA skybridge'. The DNA skybridge is constructed in a 3D structure with 4 μm-high thin quartz barriers in a quartz slide. Each DNA end is attached to the top of the adjacent barrier, resulting in the extension and immobilization of DNA. In this 3D structure, the bottom surface is out-of-focus when the target molecules on the DNA are imaged. Moreover, the DNA skybridge itself creates a thin Gaussian light sheet beam parallel to the immobilized DNA. This dual property allows for imaging a single probe-tagged molecule moving on DNA while effectively suppressing interference with the surface and background signals from the surface.
Original languageEnglish (US)
Pages (from-to)e107-e107
Number of pages1
JournalNucleic acids research
Volume47
Issue number18
DOIs
StatePublished - Jul 24 2019

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