DNA hypermethylation in somatic cells correlates with higher reprogramming efficiency

María J. Barrero, María Berdasco, Ida Paramonov, Josipa Bilic, Marianna Vitaloni, Manel Esteller*, Juan Carlos Izpisua Belmonte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The efficiency of somatic cell reprogramming to pluripotency using defined factors is dramatically affected by the cell type of origin. Here, we show that human keratinocytes, which can be reprogrammed at a higher efficiency than fibroblast [Nat Biotechnol 2008;26:1276-1284], share more genes hypermethylated at CpGs with human embryonic stem cells (ESCs) than other somatic cells frequently used for reprogramming. Moreover, pluripotent cells reprogrammed from keratinocytes (KiPS) are more similar to ESCs than those reprogrammed from fibroblasts (FiPS) in regard to DNA methylation levels, mostly due to the presence of genes that fail to acquire high levels of DNA methylation in FiPS cells. We propose that higher reprogramming efficiency correlates with the hypermethylation of tissue-specific genes rather than with a more permissive pluripotency gene network.

Original languageEnglish (US)
Pages (from-to)1696-1702
Number of pages7
JournalStem Cells
Volume30
Issue number8
DOIs
StatePublished - Aug 1 2012

Keywords

  • DNA methylation
  • Epigenetics
  • Gene expression
  • Induced pluripotent stem cells
  • Pluripotent stem cells
  • Reprogramming

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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