Diversity of auxin-producing bacteria associated to Pseudomonas savastanoi-induced olive knots

Hadda Ouzari*, Amel Khsairi, Noura Raddadi, Leila Jaoua, Abdennaceur Hassen, Mokhtar Zarrouk, Daniele Daffonchio, Abdellatif Boudabous

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Forty three strains were isolated from knots induced by Pseudomonas savastanoi in different olive cultivars. All the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (IAA). Amplification of the intergenic transcribed spacers (ITS) between 16S and 23S rDNA genes, allowed the clustering of the isolates into seven distinct groups. All isolates from ITS group 1 were positive to the Pseudomonas savastanoi pv. savastanoi specific iaaL gene as shown by PCR. Partial sequencing of 16S rDNA gene confirmed the identity of these isolates to Pseudomonas savastanoi strains and allowed to tentatively assign the other isolates from the remaining ITS groups to Pantoea oleae/agglomerans, Burkholderia cepacia, Pseudomonas putida, Stenotrophomonas maltophilia and Hafnia alvei. Identification of endophytic knot-derived isolates revealed association of various saprophytic and putative human pathogenic bacteria with P. savastanoi pv. savastanoi in knot environment of olive infected trees.

Original languageEnglish (US)
Pages (from-to)370-377
Number of pages8
JournalJournal of Basic Microbiology
Volume48
Issue number5
DOIs
StatePublished - Jan 1 2008

Keywords

  • 16S-23S intergenic spacer
  • Auxin
  • Olive knots
  • Pseudomonas savastanoi

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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