Distinct WNT pathways regulating AER formation and dorsoventral polarity in the chick limb bud

Mineko Kengaku, Javier Capdevila, Concepción Rodriguez-Esteban, Jennifer De La Peña, Randy L. Johnson, Juan Carlos Izpisua Belmonte*, Clifford J. Tabin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

349 Scopus citations

Abstract

The apical ectodermal ridge (AER) is an essential structure for vertebrate limb development. Wnt3a is expressed during the induction of the chick AER, and misexpression of Wnt3a induces ectopic expression of AER-specific genes in the limb ectoderm. The genes β-catenin and Lef1 can mimic the effect of Wnt3a, and blocking the intrinsic Lef1 activity disrupts AER formation. Hence, Wnt3a functions in AER formation through the β-catenin/LEF1 pathway. In contrast, neither β-catenin nor Lef1 affects the Wnt7a-regulated dorsoventral polarity of the limb. Thus, two related Wnt genes elicit distinct responses in the same tissues by using different intracellular pathways.

Original languageEnglish (US)
Pages (from-to)1274-1277
Number of pages4
JournalScience
Volume280
Issue number5367
DOIs
StatePublished - May 22 1998

ASJC Scopus subject areas

  • General

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