Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Jonas Hühn, Chiara Fedeli, Qian Zhang, Atif Masood, Pablo del Pino, Niveen M. Khashab, Emanuele Papini, Wolfgang J. Parak

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Protein adsorption to nanoparticles is described as a chemical reaction in which proteins attach to binding sites on the nanoparticle surface. This process can be described with a dissociation coefficient, which tells how many proteins are adsorbed per nanoparticle in dependence of the protein concentration. Different techniques to experimentally determine dissociation coefficients of protein adsorption to nanoparticles are reviewed. Results of more than 130 experiments in which dissociation coefficients have been determined are compared. Data show that different methods, nanoparticle systems, and proteins can lead to significantly different dissociation coefficients. However, we observed a clear tendency of smaller dissociation coefficients upon less negative towards more positive zeta potentials of the nanoparticles. The zeta potential thus is a key parameter influencing protein adsorption to the surface of nanoparticles. Our analysis highlights the importance of the characterization of the parameters governing protein-nanoparticle interaction for quantitative evaluation and objective literature comparison.
Original languageEnglish (US)
Pages (from-to)148-161
Number of pages14
JournalThe International Journal of Biochemistry & Cell Biology
Volume75
DOIs
StatePublished - Jan 1 2016

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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