Abstract
Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index across a sample (e.g. cell or neuron). In short,DHM added a new dimension to optical imaging,whic h explains why it is such an excellent instrument for metrological,but also for biological applications. We believe that DHM is,b y nature,ideally suited for nonlinear microscopy. In this work,w e review the advantages of DHM for nonlinear microscopy and present its application to determination of the axial position of nonlinear nanoparticles capable of second harmonic generation.
Original language | English (US) |
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Title of host publication | Biophotonics |
Subtitle of host publication | Photonic Solutions for Better Health Care II |
Volume | 7715 |
DOIs | |
State | Published - 2010 |
Externally published | Yes |
Event | Biophotonics: Photonic Solutions for Better Health Care II - Brussels, Belgium Duration: Apr 12 2010 → Apr 16 2010 |
Other
Other | Biophotonics: Photonic Solutions for Better Health Care II |
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Country | Belgium |
City | Brussels |
Period | 04/12/10 → 04/16/10 |
Keywords
- 3D-tracking
- Digital holographic microscopy (DHM)
- Digital holography
- Nanoparticles
- Nonlinear microscopy
- Second harmonic generation (SHG)
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging