Digital holographic microscopy applied to neurociences

Pierre Marquet, Daniel Boss, Pascal Jourdain, Pierre Magistretti, Nicolas Pavillon, Christian Depeursinge

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Quantitative phase imaging techniques including digital holographic microscopy (DHM) have emerged recently in life sciences and can be aimed at monitoring and quantifying, in a non-invasive way, cellular processes. Due to its interferometric nature, the DHM phase signal or optical path difference (OPD), which depends on both cell thickness and integral RI can be measured with a high sensitivity. Here, we present an application of digital holographic microscopy (DHM) dedicated towards early and label free detection of neuronal death. Practically, through the quantitative measurements of OPD, the early neuronal cell volume regulation, following glutamate-mediated excitotoxicity, has been accurately monitored. Finally, the efficiency of this early cell volume regulation process has been successfully correlated with the occurring of the subsequent neuronal death assessed with the widely accepted trypan blue viability method.

Original languageEnglish (US)
Title of host publication2012 11th Euro-American Workshop on Information Optics, WIO 2012
DOIs
StatePublished - Dec 1 2012
Event2012 11th Euro-American Workshop on Information Optics, WIO 2012 - Quebec City, QC, Canada
Duration: Aug 20 2012Aug 24 2012

Publication series

Name2012 11th Euro-American Workshop on Information Optics, WIO 2012

Other

Other2012 11th Euro-American Workshop on Information Optics, WIO 2012
CountryCanada
CityQuebec City, QC
Period08/20/1208/24/12

ASJC Scopus subject areas

  • Information Systems
  • Electronic, Optical and Magnetic Materials

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