Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics

Pierre Marquet*, Benjamin Rappaz, Tristan Colomb, Florian Charrière, Jonas Kühn, Yves Emery, Etienne Cuche, Christian Depeursinge, Pierre Magistretti

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.

Original languageEnglish (US)
Title of host publicationBiophotonics and New Therapy Frontiers
Volume6191
DOIs
StatePublished - 2006
Externally publishedYes
EventBiophotonics and New Therapy Frontiers - Strasbourg, France
Duration: Apr 3 2006Apr 5 2006

Other

OtherBiophotonics and New Therapy Frontiers
CountryFrance
CityStrasbourg
Period04/3/0604/5/06

Keywords

  • 3D cellular dynamics
  • Digital Holography Microscopy
  • Intracellular refractive index

ASJC Scopus subject areas

  • Engineering(all)

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