Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds

Ali Idris, John Richard Tuttle, Dominique Niki Robertson, Candace H. Haigler, Judith K. Brown

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A Cotton leaf crumple virus (CLCrV)-based gene silencing vector containing a fragment of the Gossypium hirsutum Magnesium chelatase subunit I was used to establish endogenous gene silencing in cotton of varied genetic backgrounds. Biolistic inoculation resulted in systemic and persistent photo-bleaching of the leaves and bolls of the seven cultivars tested, however, the intensity of silencing was variable. CLCrV-VIGS-mediated expression of green fluorescent protein was used to monitor the in planta distribution of the vector, indicating successful phloem invasion in all cultivars tested. Acala SJ-1, one of the cotton cultivars, was identified as a particularly optimal candidate for CLCrV-VIGS-based cotton reverse-genetics. © 2010 Elsevier Ltd.
Original languageEnglish (US)
Pages (from-to)13-22
Number of pages10
JournalPhysiological and Molecular Plant Pathology
Volume75
Issue number1-2
DOIs
StatePublished - Aug 4 2010

ASJC Scopus subject areas

  • Plant Science
  • Genetics

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