DHM (Digital Holography Microscope) for imaging cells

Yves Emery*, Etienne Cuche, Tristan Colomb, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Pierre Magistretti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Light interaction with a sample modifies both intensity and phase of the illuminating wave. Any available supports for image recording are onlysensitive to intensity, but Denis Gabor [P. Marquet, B. Rappaz, P. Magistretti, et. al. Digital Holography for quantitative phase-contrast imaging, Optics Letters, 30, 5, pp 291-93 (2005)] invented in 1948 a way to encode the phase as an intensity variation: the "hologram". Digital Holographic Microscopy (DHM) [D. Gabor, A new microscopic principle, Nature, 1948] implements digitally this powerful hologram. Characterization of various pollen grains and of morphology changes of neurones associated with hypotonic shock demonstrates the potential of DHM for imaging cells.

Original languageEnglish (US)
Article number260
Pages (from-to)1317-1321
Number of pages5
JournalJournal of Physics: Conference Series
Volume61
Issue number1
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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