Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin

Laszlo Bögre, Claudia Jonak, Mátyás Mink, Irute Meskiene, Jan Traas, Dang Thi Cam Ha, Ines Swoboda, Christian Plank, Ernst Wagner, Erwin Heberle-Bors, Heribert Hirt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

We report here the isolation and characterization of the nucMs1 alfalfa cDNA, whose predicted amino acid sequence structurally resembles the yeast Nsr1 protein and animal nucleolins. These proteins consist of an N-terminal acidic domain, centrally located RNA recognition motifs (RRMs), and a C-terminal glycine- and arginine-rich domain. In comparison with animal nucleolins that contain four RRMs, NucMs1 more closely resembles the yeast Nsr1 protein, which contains only two RRMs. A NucMs1 C-terminal peptide antibody specifically recognized a 95-kD nucleolar protein in alfalfa cells that changed its localization in a cell cycle-dependent manner. The nucMs1 transcript and p95 nucMs1 protein levels correlated with cell proliferation, and nucMs1 gene expression was found to be induced in the G 1 phase upon mitogenic stimulation of G 0 -arrested leaf cells. In situ hybridization analysis of different alfalfa organs during various developmental stages showed that nucMs1 gene expression is highest in root meristematic cells, but it is also found in other meristematic cells of the plant body. nucMs1 expression is tightly linked to cell proliferation but does not depend on a particular cell cycle phase. No nucMs1 expression was observed in cells that had exited the cell cycle and were undergoing differentiation or polar growth, indicating that nucMs1 may not be necessary for processes other than cell proliferation.

Original languageEnglish (US)
Pages (from-to)417-428
Number of pages12
JournalPlant Cell
Volume8
Issue number3
StatePublished - Mar 1 1996

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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