Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system

Seung Soo Han, Jinjoo Lee, Yideul Jung, Myeong Ho Kang, Jung Hyub Hong, Min Suk Cha, Yu Jin Park, Ezra Lee, Cheol Hee Yoon, Yong Soo Bae*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.

Original languageEnglish (US)
Article number16768
Pages (from-to)4827-4836
Number of pages10
JournalVaccine
Volume33
Issue number38
DOIs
StatePublished - Sep 11 2015
Externally publishedYes

Keywords

  • HIV-1 p24
  • Oral CTL vaccine
  • P24-specific CTL
  • Poliovirus Sabin 1 vector
  • Recombinant poliovirus
  • RPS-CTP CTL vector

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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