Development of a sensing system to detect C-telopeptide of type-I collagen

Nasrin Afsarimanesh, Asif I. Zia, S. C. Mukhopadhyay, Marlena Kruger, P. L. Yu, Jürgen Kosel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.
Original languageEnglish (US)
Title of host publication2015 9th International Conference on Sensing Technology (ICST)
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages586-590
Number of pages5
ISBN (Print)9781479963140
DOIs
StatePublished - Mar 30 2016

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