Detection of antibiotic resistance in probiotics of dietary supplements

Aloysius Tze Wong, Davey Yueh Saint Ngu, Lydia Annabel Dan, Amanda Siok Lee Ooi, Renee Lay Hong Lim

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Background Probiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteria act as reservoir for antibiotic resistant determinants, the transfer of these genes to pathogens sharing the same intestinal habitat is thus conceivable considering the fact that dietary supplements contain high amounts of often heterogeneous populations of probiotics. Such events can confer pathogens protection against commonly-used drugs. Despite numerous reports of antibiotic resistant probiotics in food and biological sources, the antibiogram of probiotics from dietary supplements remained elusive. Findings Here, we screened five commercially available dietary supplements for resistance towards antibiotics of different classes. Probiotics of all batches of products were resistant towards vancomycin while batch-dependent resistance towards streptomycin, aztreonam, gentamycin and/or ciprofloxacin antibiotics was detected for probiotics of brands Bi and Bn, Bg, and L. Isolates of brand Cn was also resistant towards gentamycin, streptomycin and ciprofloxacin antibiotics. Additionally, we also report a discrepancy between the enumerated viable bacteria amounts and the claims of the manufacturers. Conclusions This short report has highlighted the present of antibiotic resistance in probiotic bacteria from dietary supplements and therefore serves as a platform for further screenings and for in-depth characterization of the resistant determinants and the molecular machinery that confers the resistance.
Original languageEnglish (US)
JournalNutrition Journal
Volume14
Issue number1
DOIs
StatePublished - Sep 14 2015

Fingerprint Dive into the research topics of 'Detection of antibiotic resistance in probiotics of dietary supplements'. Together they form a unique fingerprint.

Cite this