Detection methods for the viability and infectivity of Cryptosporidium oocysts

Weiwei Li, Zhimin Qiang*, Yanwei Shi, Tao Zhang, Shuhang Wu

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

Abstract

Methods for determining the viability and infectivity of Cryptosporidium oocysts after inactivation by disinfectants, have received much attention. In this paper, five commonly used methods, namely animal infectivity, cell culture, fluorogenic vital dyes, in vitro excystation and reverse transcription polymerase chain reaction (RT-PCR), are reviewed to provide useful information for choosing and optimizing the detection methods. The research process, applicability, reliability, and advantages and disadvantages are systematically discussed.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalHuanjing Kexue Xuebao/Acta Scientiae Circumstantiae
Volume30
Issue number3
StatePublished - Mar 1 2010

Keywords

  • Animal infectivity
  • Cell culture
  • Cryptosporidium
  • Fluorogenic vital dyes
  • In vitro excystation
  • Infectivity
  • RT-PCR
  • Viability

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Environmental Science(all)

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